Terminator

Part:BBa_K2715014

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


Terminator.png

HydA Terminator from C.acetobutylicum


Usage


The hydA transcriptional terminator was used in two composite parts BBa_K2715007 and BBa_K2715008 which aimed to suppress toxin production in C. difficile. The composite parts consisted of two inhibitory antisense RNA parts which the hydA terminator was responsible for breaking up into two separate asRNA molecules by terminating transcription.

Biology


This terminator part is native to C. acetobutylicum where it lies between the two genes pyrE and hydA.


Secondary Structure

BBa K2715014 pic.png

Measurement
This terminator had been previously shown to function by our host research group SBRC nottingham, and it was used in the two composite parts Bba_K2715007 and Bba_K2715008.

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Parameters
None